方法:本研究利用CRC患者手术标本、大肠组织相关癌症小鼠模型、结肠类器官和共培养细胞系,检测不同病理区域CD11b/CD86的变化,并检测Wnt信号通路的活性。 结果:我们的发现显示,从早期到晚期CRC组织,CD11b的表达敏感且增加,与预后不良相关,而晚期CRC组织中CD86的表达降低。CD133在晚期CRC组织中的表达也升高,主要与CD11b...
Results Our findings revealed a sensitive and increased expression of CD11b from the early to the advanced CRC tissues and correlated with poor prognosis, while CD86 expression was reduced in advanced CRC tissues. CD133 expression was also elevated in advanced CRC tissues and mainly co-...
与M1极化相关的遗传标志物包括IL1a、IL1b、IL6、NOS2、TLR2、TLR4、CD80和CD86。 替代活化型M2巨噬细胞 对于M2型巨噬细胞,激活是通过IL4、IL10和IL13等细胞因子。值得注意的是,M1和M2表型是连续的,因此处于中间范围的巨噬细胞可以同时表达一些标志物。 与经典活化亚型相反,替代活化的亚型表达相反的表达谱:...
刺激24小时后,用流式的方法检测极化后M1、M2标志蛋白的表达。M1巨噬细胞上调表达CD86、MHCⅡ、CD11c等,M2巨噬细胞上调表达CD206等; 实验结果举例: 参考文献: 1. JoVE. June 2013 |https://doi.org/10.3791/50323 2. Sci Rep. 2020 |https://doi.org/10.1038/s41598-020-57677-5 THP-1细胞向M1,M2的...
此外,通过调节TAM中的CD11b信号传导,GB1275能够增加干扰素相关因子(IFNα1、IFNβ、CXCL9和CXCL10)和抗原呈递/共刺激复合物(MHC-I、MHC-II、CD80和CD86)的表达水平,同时降低免疫抑制基因(IL10、TGFβ和精氨酸酶-1)的表达,因此,GB1275对CD11b的变构调节导致TAM极化向促炎和抗原呈递表型的转变,增强干扰素反应并...
Here, we report that small hairpin RNA (shRNA) mediated LSD1 inhibition causes a remarkable transcriptional activation of myeloid lineage marker genes (CD11b/ITGAM and CD86), reduction of cell proliferation and decrease of clonogenic ability of human AML cells. Cell surface expression of CD11b ...
A second subset of CD11cnegCD11bpos immune cells (subset E; Figure 2a) was also present in Mem71-infected and HckF/F mouse BAL, which stained negatively for myeloid markers Class II, CD14, or CD86 (Figure 2b). Cell differentials using BAL cytospins, cell sorting, and Siglec F staining ...
CD86, Rat Anti-Mouse, FITC CD90 Thy-1 Antibody Thy-1 (CD90), Rat Anti-Mouse CD90 Thy-1.1 Antibody Thy-1.1 (CD90), Rat Anti-Mouse CD90 Thy-1.2 Antibody Thy-1.2 (CD90), Rat Anti-Mouse Anti Viral Dengue type 2 Antibody Dengue Type 2 (envelop), Mouse antibody ...
12-0869-73 PE anti-human CD86 (B7-2, B72) 100 tests 11-0114-85 FITC anti-mouse CD11c (Integrin aX, p150/90) 500 ug 17-0191-82 APC anti-mouse CD19 100 ug 16-5896-85 Functional Grade Purified anti-mouse NKG2A/C/E 500 ug 12-5981-83 PE anti-mouse Ly-6A/E (Sca-1, Sca1)...
结果:用 ADH-503 (GB1275)处理后,巨噬细胞的 MHCI、MHCII、CD80 和 CD86 表达水平更高,表明剩余巨噬细胞的抗原呈递特性有所改善。[1] 体内活性 方法:在大鼠和 C57/B6 小鼠中,给予ADH-503 (GB1275) (30,100mg / kg,口服)测试药代动力学 (PK) 和安全性。