黑框圈出的细胞群为Ly6GhighG-MDSC,红框圈出的细胞群为Ly6GlowG-MDSC(图4A1和B1)。4T1和4T07对照组Ly6GlowG-MDSC亚群的百分比分别为(13.8±2.0)%和(14.2±2.7)%,而禁食组的比例分别降至(6.2±4.7)%和(4.5±3.8)%(图4A2和B...
骨髓来源的抑制性细胞(Myeloid-derived suppressor cells，MDSCs)是骨髓来源的一群异质性细胞，是树突状细胞(dendritic cells，DCs)、巨噬细胞和(或)粒细胞的前体，具有显著抑制免疫细胞应答的能力。小鼠MDSCs的表型为CD11b+Gr-1+，分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和单核细胞样的MDSCs...
为进一步明确具体哪一类免疫细胞参与此过程,作者应用流式细胞技术分析了Ang II灌注的动脉中炎症细胞,结果表明CD45+CD11b+Ly6C+Ly6G-F4/80+巨噬细胞呈现时间依赖性地增加。这些结果提示,骨髓巨噬细胞中CD11b的表达上调可能参与高血压的发生发...
在进行细胞表面分子染色前,用CD16/CD32抗体孵育细胞悬液封闭细胞表面的Fc受体。用于检测细胞表面蛋白的抗体包括:购自ebioscience的CD3e、CD4、CD8a、CD19、NK1.1、F4/80、CD45、CD11b、CD45.1、CD45.2、Ki67、Ly6C和购自biolegend的CD115、Ly6G、和Siglec-F。IL4Rα抗体购自R&D。 通过在消化前以和胶原酶为1:...
请问组织中LY6C+CD11b+LY6G-是什么细胞?... 请问组织中LY6C+CD11b+LY6G-是什么细胞? 展开 网友 1 最佳答案 回答者:网友 骨髓来源的抑制性细胞(Myeloid-derived suppres洋战x行sor cells,MDSCs)是骨髓来源的一群异质性细胞,是树突状细胞(dendritic cells,DCs)、巨噬细胞和(或)粒细胞的前体,具有显著抑制免富...
We also found CD11b+Ly6g+ cells are major source for IFN-α. In the current study, using single cell RNA sequencing, we further characterized IFN-α producing CD11b+Ly6g+ cells in the lungs of Mtb infected alcohol-diet fed mice. Our analysis revealed '6' distinct clusters in CD11b+Ly6g...
The finding that a population of proliferating CD11b(+)Ly6G(-) myeloid cells contribute to mechanical inflammatory pain provides a potential cellular target for its treatment in wound inflammation. 展开 关键词: pain, inflammation, cytokines, monocytes, macrophages ...
RESEARCH ARTICLE Open AccessCD11b + Ly6C ++ Ly6G - cells show distinct functionin mice with chronic inflammation or tumorburdenEva Källberg, Martin Stenström, David Liberg, Fredrik Ivars and Tomas Leanderson *AbstractBackground: S100A9 has been shown to be important for the function of so ...
To this end, we sorted CD11b+ cells using FACS with regard to the expression of the Ly6G and Ly6C markers into three populations; Ly6C+G+, Ly6C+G- and Ly6C++G-, as shown in Figure 1A. RNA was prepared from the isolated cell populations and S100A9 expression was measured using Q-...
Ly6g-phycoerythrin was then used to negatively gate out all neutrophils, and all lymphocytes were gated out based on their lower FSC-A and SSC-A profile. This allowed FACS analysis on the remaining M^/monocyte subpopulations. AMFs were discriminated from dendritic cells based on their high auto...