The estimated secondary structure fractions of rice glutelin from CD spectrum shows that rice glutelin mainly composed of 锟 -form secondary structure (59.1%) followed by random coil (33.0 %) and a-helix (7.9 %). A negative minima occurred around at 209 nm accompanied by a weak shoulder at...
random coil 溶菌酶是研究高温可逆性失活的常用模型,因为其廉价、易变 性,并且其荧光光谱复杂。 溶菌酶是鸡蛋蛋白中的一种小的球蛋白,有129个氨基酸,4个 链内二硫键。主要由 α螺旋和部分ß 折叠组成。有两个色氨酸, 一个暴露,一个埋在内。 Hen egg-white lysozyme (1mg) was dissolved...
NearUVCDspectrum 蛋白质中芳香氨基酸残基，如色氨酸(Trp)、酪氨酸(Tyr)、苯丙氨酸(Phe)及二硫键处于不对称微环境时，在近紫外区250～320nm，表现出CD信号。Phe残基:255、261和268nm附近；Tyr残基:277nm左右；而在279、284和291nm是Trp残基的信息；二硫键的变化信息反映在整个近紫外CD谱上。近紫外CD谱可作为...
Alpha-helix,beta-sheet,andrandomcoilstructureseachgiverisetoacharacteristicshapeandmagnitudeofCDspectrum. Thisisillustratedbythegraphtotheright,whichshowsspectraforpoly-lysineinthesethreedifferentconformations.Theapproximatefractionofeachsecondarystructuretypethatispresentinanyproteincanthusbedeterminedbyanalyzingitsfar-UV...
Near UV CD spectrum of Lysozyme260270280290300310-200-1000100 nmMain CD features o 6、f protein 2ndary structures - band (nm)+ band (nm)-helix 222208192-sheet216195-turn220-230 (weak)180-190 (strong)205polypro II helix190210-230 weakRandom coil200212Far UV CD spectra of poly-L-LysCD ...
The IR spectrum of indolicidin in DPC micelles displays a combination of turn and random coil bands (Fig. 2, Table 2). A large increase in the intensity of Arg side chain bands (especially the symmetric vibration) is seen in the DPC spectrum compared to the buffer. The CD spectrum shows...
This spectrum is the sum ove... ME Holtzer,S Kumar,A Holtzer,... - 《Biopolymers》 被引量: 11发表: 2010年 ??-Helix-to-random-coil transition of two-chain, coiled coils. Theory and experiments for thermal denaturation of ??-tropomyosin at acidic pH Not Available ME Holtzer,A Holtzer,...
Experimentalists interested in obtaining spectroscopic measurements of membrane protein secondary structure must keep in mind one general critical issue: the measurements must be reliable and specific enough to help distinguish between competing models o
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A group of closely related bacteriostatic antibiotics, with similar antibacterial spectrum and toxicity. They act by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis. Effective against many streptococci, Gram-negative bacilli, rickettsiae, spirochetes, Mycoplasma, and ...