The far UV spectrum (panel A) and near UV spectrum (panel B) show that the wild-type (solid line) and mutant (dotted line) enzymes have very similar secondary and tertiary structures. The loss of catalytic activity in the mutant cannot therefore be due to an inability to acquire the corr...
9 μg of oligomeric Aβ was loaded into pre-cast 4–20% Tris-Glycine gradient gel (Bio-Rad, 4561096) and transferred to nitrocellulose membrane (Cytiva, 10600004). Membrane was blocked with 5% BSA in TBST and incubated with anti-Aβ antibody (Clone...
To this end, we used a 29-marker multiplex CyTOF panel to immunophenotype a broad spectrum of immune cells like T, B and NK lymphocytes, myeloid cells as well as their subpopulations (Fig. 4). Initially, we performed clustering with FlowSOM algorithm to identify and annotate 31 distinct ...
9 μg of oligomeric Aβ was loaded into pre-cast 4–20% Tris-Glycine gradient gel (Bio-Rad, 4561096) and transferred to nitrocellulose membrane (Cytiva, 10600004). Membrane was blocked with 5% BSA in TBST and incubated with anti-Aβ antibody (Clone...
By analyzing the fluorescence spectrum and intensity, it was observed that the DBP had a strong ability to quench the intrinsic fluorescence of BSA through a static quenching procedure. The binding constantsdoi:10.1080/10934529.2022.2136909Xin.Wang...
4. Can CD206 marker be used as a marker of M2 macrophages? The activation of macrophages with various stimuli leads to their polarization into classical (M1) or alternatively activated (M2) subtypes spectrums and both subtypes differ in their regulatory and effector functions (PMID: 24669294). ...
CD147, also named extracellular matrix metalloproteinase inducer (EMMPRIN), is well known as a potent inducer of MMPs. To determine whether CD147 enhanced collective invasion was dependent entirely on MMPs induction, we treated cells with a broad-spectrum MMPs inhibitor TAPI-2 and found that CD...
Brain sections were photobleached in a 4.5% (w/v) H2O2 and 20 mM NaOH in PBS solution for 2 h followed by incubation in PBS under broad-spectrum LED lights for 48h (ex/Aibecy A4 Ultra Bright 25,000 Lux LED Light Box-Tracing Pad). The photobleaching protocol was adapted from ...
NAFLD is considered to be the liver manifestation of metabolic syndrome, which encompasses a wide spectrum beginning with steatosis (2, 3). The cluster of differentiation 36 (CD36) belongs to the scavenger receptor family, also known as fatty acid translocase, that facilitates the uptake of long...
Cancer-associated fibroblasts (CAFs) are abundantly present in the microenvironment of virtually all tumors and strongly impact tumor progression. Despite increasing insight into their function and heterogeneity, little is known regarding the origin of CAFs. Understanding the origin of CAF heterogeneity is...