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To determine whether neonatal eosinophils have a quantitative impairment in Mac-1 up-regu- lation similar to that of neonatal neutrophils, we developed a method for measuring Mac- 1 on neutrophils and eosinophils in mixed leukocyte suspensions using dual-labeling with an anti- CD16 MAb together ...
Rat TA1 p900, a fragment corresponding to nucleotides 816–1536 of the full-length rat LAT-1, inserted into Bluescript SK vector was labeled with [32P]dCTP (NEN Life Science Products; 3000 Ci/mmol) by random primed labeling (Roche Molecular Biochemicals) for use as a probe. Blots wrapped...
Do not change the ‘Input’ (first row) labeling. Select ‘Yes’ or ‘OK’ for any Excel questions about saving in this format. 'RunVars_multispecies.csv' provides example inputs for 2 species, and 'RunVars_3species.csv' provides example inputs for 3 species. Start the program: For ...
140-000-059.05 This MACS product is for in vitro research use only ® and not for diagnostic or therapeutic procedures. page 2/4 Order No. 130-049-601 3. Apply cell suspension onto the column. ▲Note: Program choice depends on the isolation strategy, the strength of magnetic labeling ...
open at your own discretion. I have opened all files on the drive through the notepad and then copied into word doc with headers labeling what file the content originated from. This should be enough to not compromise your system while viewing, but I am no expert and cannot g...
Isolated RNA from antibody-purified cell fractions (CD4, CD8, CD14, CD19, CD56) from 19 individuals (9 smokers, 10 nonsmokers (See Table S1)), was amplified using NuGEN WT-Ovation Pico RNA Amplification System followed by labeling with NuGEN Encore Biotin Module according to the manufacturer...
Optional labeling of the pseudoge- nomes with 5-ethinyl-2′- deoxyuridine (EdU) was performed by supplementing the growth medium with 100 μM EdU 6 h post transfection79. Statistics. All experiments were reproduced at least three times. All analysable data points were included in ...
The terms also encompass an amino acid polymer that has been modified naturally or by intervention; for example, disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation or modification, such as conjugation with a labeling component. Also included ...