Genome wide expression analysis of the CCR4-NOT complex indicates that it consists of three modules with the NOT module controlling saga-responsive genes. Mol. Genet. Genomics 297, 323–337.Cui Y, Ramnarain DB, Chiang YC, Ding LH, McMahon JS, et al. (2008) Genome wide expression analysis...
The core CCR4-NOT complex elutes in fractions 7–14. Data from one representative experiment are shown (left panel), and the relative distribution of RNF219 and NOT7 along the gradient was quantified (right panel); values are presented as mean ± SD (WT n = 3; ΔSLiM n =...
The CCR4-NOT complex is one of two major multi-subunit poly(A) deadenylation complexes, which mediates the shortening of the poly(A) tails and starts the mRNA decay process29,30,31,32. The CCR4-NOT complex can be recruited to specific target mRNAs via its interaction with the RNA-inducing...
2. Description of the Ccr4-Not complex 2.1. Composition The Ccr4-Not complex is evolutionarily conserved from yeast to human [21], [22], [23], [24], [25]. In Saccharomyces cerevisiae the core of the Ccr4-Not complex has a size of about 1 MDa and comprises 9 proteins (5 Not protein...
To further validate the interaction between CCR4-NOT complex and MARTRE1/2 proteins, we generated HEK293 cell lines stably expressing FLAG-tagged MARTRE1 or MARTRE2 and performed affinity purification with antibodies against FLAG. Indeed, all core subunits of the CCR4-NOT complex were identified in...
Thus, with respect to the evolutionarily conserved core of the CCR4-NOT complex (four NOT proteins and the two catalytic subunits), structural information is currently lacking for the N-terminal coiled coil domain of NOT3 and the connector domain of NOT1. Here, we present the crystal structure...
DND1 maintains germline stem cells via recruitment of the CCR4–NOT complex to target mRNAs Yamaji, M., et al., DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature, 2017. 543(7646): p. 568-572.Yamaji, M. et al. DND1 maintains germline ...
DND1 maintains germline stem cells via recruitment of the CCR4–NOT complex to target mRNAs Yamaji, M., et al., DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature, 2017. 543(7646): p. 568-572.Yamaji, M. et al. DND1 maintains germline ...
Bic-C binds specific segments of the Bic-C 5鈥 untranslated region and negatively regulates its own expression by binding directly to NOT3/5, a component of the CCR4 core deadenylase complex, thereby promoting deadenylation. Bic-C overexpression induces premature cytoplasmic-streaming, a posterior...
Here we show that in mice DND1 binds a UU(A/U) trinucleotide motif predominantly in the 3′ untranslated regions of mRNA, and destabilizes target mRNAs through direct recruitment of the CCR4–NOT deadenylase complex. Transcriptomic analysis reveals that the extent of suppression is dependent on...