However, these studies used less precise phenotyping strategies and used intense depletion of endo- genous myeloid cells that may alter the fate of donor cells and their progeny.19–21,23 In our hands, Ly6Chi monocytes were unable to generate CD103 À CD11b þ DCs and gave rise only to...
Figure 4. CCR2 expression defines two populations of intestinal CD103−CD11b+ dendritic cells (DCs). (a) Representative CCR2 staining on CD11c+MHCII+CD64− DC populations from small intestinal lamina propria (SI LP) of wild-type (WT) mice, compared with total DCs from CCR2−/− ...
43 Also, activation of alloreactive CCR2-/- CD8+ T cells as determined by CD44 up-regulation on the fast-proliferative allo-activated population was comparable to WT CD8+ T-cell activation (Figure 4C). These data were confirmed by examining the percentage of activated (CD44hiCD62Llow) donor...
. (H) The immune cell (CD45+) infiltrations in the three groups were statistically analyzed. (I) The percentages of macrophages (F4/80+) among all of the immune cells in the cLP of the three groups were statistically analyzed. (J–L) The myeloid-derived cells (CD11b+) and granulocytes...