No. CCILegal Observer J Jurisprudence
El primer estudio prospectivo multicéntrico de la prevalencia de la ATTR-AC en pacientes de edad avanzada con IC y cualquier valor de fracción de eyección se publicó en 202318. No hay estudios multicéntricos en los que se haya evaluado la prevalencia de la ATTR-AC en pacientes con...
Briefly, the DNA was mixed with the primer pool, containing all primers for generating the 224 amplicons and the AmpliSeq HiFi Master Mix, and transferred to a PCR cycler (BioRad, Munich, Germany). PCR cycling conditions were as follows: Initial denaturation: 99°C for 2 min, cycling: ...
0.5 to 2 μg mRNA was reverse transcribed in a reaction mix containing 10U/μL Moloney Murine Leukemia Virus reverse transcriptase (Life Technologies), 5 ng/ μL Random Primers (Life Technologies), 1x first strand buffer (Life Technologies), 5 mM DTT (Life Technologies)...
(stratec, birkenfeld, germany). amplicon library preparation was performed using approximately 10ng of dna as advised by the manufacturer. briefly, the dna was mixed with the primer pool, containing all primers for generating the 224 amplicons and the ampliseq hifi master mix, and transferred to...