Briefly, the DNA was mixed with the primer pool, containing all primers for generating the 224 amplicons and the AmpliSeq HiFi Master Mix, and transferred to a PCR cycler (BioRad, Munich, Germany). PCR cycling conditions were as follows: Initial denaturation: 99°C for 2 min, cycling: ...
Real-time-PCR was performed in a reaction volume of 20 μl containing 2 μl cDNA, Light Cycler TaqMan Master (Roche), primers and probes for p21, EGFR and the housekeeping gene porphobilinogen deaminase (PBGD) in concentrations recommended by the manufacturer (Real Time Ready Assays, Roche...
INTRODUCTION Leukemia characterised by rearrangement of the mixed lineage leukemia (MLL) gene is a highly aggressive cancer that occurs in over 70% of infant acute lymphoblastic leukemia (ALL) and 35-50% of infant acute myeloid leukemia (AML) [1, 2]. Notwithstanding the signi...
(FEVI) ≥ 50%, altas concentraciones de péptidos natriuréticos (BNP > 35 pg/ml o NT-proBNP > 125 pg/ml) y una cardiopatía estructural relevante (HVI, agrandamiento de la aurícula izquierda) o disfunción diastólica; al menos 1 ingreso previo por IC en los últimos 24 meses y ...