本发明还公开了具有ccdB负筛作用的菌株构建方法,包括以下步骤:(1)利用Red/ET同源重组技术将菌株中F因子上的ccdA和TetR基因替换为FRT‑Kan cassette‑FRT DNA序列,得到基因编辑后的F因子;(2)提供表达FLP重组酶的质粒,通过所述质粒表达的FLP重组酶介导重组反应,删除所述基因编辑后的F因子上两个FRT位点中间的外源...
(2)提供表达FLP重组酶的质粒,通过所述质粒表达的FLP重组酶介导重组反应,删除所述基因编辑后的F因子上两个FRT位点中间的外源序列(即Kan cassette),即得所述具有ccdB负筛作用的菌株。需要说明的是,构建的菌株为大肠杆菌克隆菌株NEB Stable(△ccdA/B),该菌株具有ccdB负筛作用,可应用于基于Gateway和Golden Gate技术的...
本发明公开了一种具有ccdB负筛作用的菌株,所述菌株为去掉了F因子中ccdA基因和TetR基因的菌株.本发明还公开了具有ccdB负筛作用的菌株构建方法,包括以下步骤:(1)利用Red/ET同源重组技术将菌株中F因子上的ccdA和TetR基因替换为FRTKan cassetteFRT DNA序列,得到基因编辑后的F因子;(2)提供表达FLP重组酶的质粒,通过所...
牛津词典附录中的3000个释义词汇 1.abbreviate 2.accuse 3.adjective 4.administrative 5.adverb 6.aggressive 7.alphabet 8.amuse 9.ankle 10.a a nniversary 10.11.a a ppropriate 11.12.a a pproval 12.13.13.a a rch 14.a a rtificial 14.15.a a utomatically 15.1.bacteria 2.beam 3.bean ...
Said method comprises the following steps: (1) replacing ccdA and TetR genes in the F factor in NEB® Stable strain with FRT-Kan cassette-FRT DNA sequence by using Red/ET homologous recombination technology to obtain a gene edited F factor; and (2) providing a plasmid expressing FLP ...
参与胆汁酸转运的蛋白包括:ABC(ATP-binding cassette)转运蛋白、胆盐输出泵(bile salt export pump,BSEP)、多耐药相关蛋白(multidrug resistance-associated protein,MRP)2等,它们均位于毛细胆管膜上[9]。 BSEP是肝细胞胆汁酸盐输出系统中起主要作用的...
Mammographic imaging with a small format CCD-based digital cassette: Physical characteristics of a clinical system The physical characteristics of a clinical charge coupled device (CCD)-based imager (Senovision, GE Medical Systems, Milwaukee, WI) for small-field digital... V Srinivasan,K Andrew,S...
Integrons, have been described in the 90's and contain one or more gene cassettes located at a specific site. Integrons are defined by an intI gene encoding an integrase, a recombination site attI and a strong promoter. A gene cassette includes an open reading frame and, at the 3′-end,...
VARIAN 0748100BLK, HINGE CASSETTE DR VARIAN 0748200CATCH CASSETTE VARIAN 0748500GEAR CARRIAGE WORM VARIAN 0748600WAFER SLIDE GLASS VARIAN 0751400PLATEN GEAR MOD VARIAN 0751900LIGHT ROD VARIAN 0756200SPACER, INSULATOR VARIAN 0756300ROTARY DRIVE SHAFT VARIAN 0757200BUSHING VARIAN 0757700STOP TAB PLATTEN VARIAN...
Digital sensor cassette for mammography A CCD-scintillator x-ray image sensor (18) has a high sensitivity at room temperature and a low profile, enabling the use of the x-ray image sensor in most modern mammography x-ray machines. A cassette 10 that encloses the CCD-based x-ra... M Sa...