In the present study, a CBS domain containing protein gene, OsCBSX3, is functionally characterized in rice resistance against Magnaporthe oryzae (M. oryzae). By quantitative real-time PCR, transcripts of OsCBSX3 are up-regulated significantly by inoculation of M. oryzae and the exogenously ...
(b)Model of the potential arrangement of the cytoplasmic domains with respect to the transmembrane channel. The dimer structure of the domains was modeled on the basis of the known structure of a bacterial CBS domain-containing protein. The orientation with respect to the channel is unknown. The...
ANI_1_76154 Zinc-binding alcohol dehydrogenase domain-containing protein cipB protein-coding ANI_1_1132134 hypothetical protein protein-coding ANI_1_434034 protein qutG protein-coding ANI_1_2086064 WD repeat protein protein-coding ANI_1_1570184 DNA mismatch repair protein (Mlh3) protein-coding ANI...
The exchange of the vector backbone of pRE- OpuA to pERL, containing a L. lactis origin of replication, was performed as described previously (Geertsma, Poolman 2007), resulting in pNZOpuA S24C+X###C his where (X= residue in CBS domain/anionic tail of OpuAA; # = position of the ...
The MAT loci are regions of the genome which contain one or more open reading frames of which at least one encodes a transcription factor [13,14]. Conventionally, the MAT locus containing a transcription factor with an α1 domain similar to the MATα1 ofS. cerevisiaeis called MAT1–1, wh...
A third group consists of glycosyl phosphatidylinositol– anchored enzymes, which may play a role in the maintenance of the cell walls containing a-glucan38. The A. niger genome contains 42 enzymes classified as CAZy family members without a signal sequence. The majority of these are likely to ...
Medium containing Yeast extract, Peptone and Xylose rpm: Revolutions per minute PCR: Polymerase chain reaction RTq-PCR: Quantitative reverse transcriptase polymerase chain reaction CDW: Cell dry weight OD: Optical density HPLC: High pressure liquid chromatography PI: Propidium iodide DHR-123...
Total protein was extracted using RIPA buffer and the extracts containing equal quantities of protein (30 μg) were electrophoresed in 10% polyacrylamide gel, transferred with PVDF membranes and blocked for 1 h (5% BSA in TBS-Tween 20 buffer) at room temperature. Incubations with primary antib...
Hybrid four-CBS-domain KINβγ subunit functions as the canonical gamma subunit of the plant energy sensor SnRK1 (SNF1-related kinase 1) [17]. OsCBSX4, a CBS domain containing protein in rice, was found that its over-expression in transgenic tobacco plants improved tolerance to salt, heavy...
International Journal of Molecular Sciences Article Identification and Comparative Analysis of CBS Domain-Containing Proteins in Soybean (Glycine max) and the Primary Function of GmCBS21 in Enhanced Tolerance to Low Nitrogen Stress Qingnan Hao 1,2, Weijuan Shang 1,2, Chanjuan Zhang 1,2, Haifeng...