acetyltransferase activity of p300BRPHZTtoward the H2B-tetra-acetylated nucleosome. Columns marked ac (in red) indicate the H2BK12/K15/K20/K23-acetylated nucleosome. Data are mean ± SD from three independent experiments.Pvalue was calculated by a two-sample one-sided Welch’sttest. The ...
1Research Institute Children's Cancer Center Hamburg, Martinistrasse 52, N63 (HPI), 20251 Hamburg, Germany 2Department of Pediatric Hematology and Oncology, University Medical Center, Hamburg-Eppendorf, 20246 Hamburg, Germany Full list of author information is available at the end of the article ...
Download: Download full-size image Fig. 2. Defects in regeneration and neoblast pool and proliferation after cbp-2 and cbp-3 silencing. (A, E) Live cbp-2(RNAi) (A), cbp-3(RNAi) (E) and gfp(RNAi) animals. Regenerating trunk pieces from cbp-2(RNAi) animals fail to form a proper ...
Full size image Next, we analyzed the effect of the stereoisomers on CIC-DUX4 and its target genes in cells cultured with the compounds for 24 h. Except for A-485 at the highest concentration (0.3 µM), none of the compounds significantly affected CIC-DUX4 expression (Fig. 3C)...
100% acetylation of full-length HMG I (1–107) corresponds to 12,000 cpm for CBP HAT and 14,000 cpm for P/CAF HAT. (B) The same derivatives shown in (A) were acetylated by P/CAF HAT, separated by SDS PAGE, and detected by autoradiography. Some of the HMG I derivatives display ...
(c) Huh7 cells were transfected with plasmids expressing full-length PCAF mRNA or empty control plasmid, and Huh7 Control cells and Huh7 PCAF cells were treated with 5 mM 3-MA or 50 nM bafilomycin A1 for 24 h. Cell lysates were analyzed by immunoblotting for LC3 II and p62. In Huh7...
Full size image To further determine the effect of PCAF on the cell autophagy of HCC cells, the expression of PCAF in Hep3B cells was silenced. Figure 2a showed that PCAF expression was decreased significantly by siRNAs transfection in Hep3B cells. Moreover, we observed attenuated accumulation ...
1. Pictures of Lab glassware flat bottom glass test tube chemical test tube 2. Specification of Lab glassware flat bottom glass test tube chemical test tube a. Material: High quality glass b. Different sizes 12*75mm 12*100mm 13*100mm 16*125mm 16*150mm... c. ...
Download: Download full-size image Fig. 5. The effect of small molecule inhibitors on direct cardiac reprogramming. Flow cytometry analysis showing the effect of (a) HMBA and SAHA (n = 3), (b) (−)-JQ1, (+)-JQ1, I-BET 762 and RVX-208 (n = 3), (c) CBP30, I-CBP112 and ...
including mammals such as primates (e.g. humans) and rodents (e.g., mice and rats), unless otherwise indicated. The term encompasses “full-length,” unprocessed CBP as well as any form of CBP that results from processing in the cell. The term also encompasses...