CBP Form 3461- Instructions (10/12) CBP Form 3461 - Instructions DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection BLOCK 1 - ARRIVAL DATE Record the month, day, year (MM/DD/YYYY) the goods arrived at a U.S. port of entry. For pre-filed entries, use the scheduled ...
Customs and Border Protection CBP FORM 7501 INSTRUCTIONS (Updated July 24, 2012) BLOCK 1) ENTRY NUMBER Record the 11 digit alphanumeric code. The entry number is comprised of the three digit filer code, followed by the seven digit entry number, and completed with the one digit check digit....
Total RNA was extracted from 0.5–1 x 106 S2 cells and mESCs using TRIzol LS (Ambion) and purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen) according to the manufacturer’s instructions. To obtain nuclear RNA, S2 cells were resuspended in buffer A (10mM Tris-HCl pH...
CBPFORM7501INSTRUCTIONS BLOCK1)ENTRYNUMBER Recordthe11digitalphanumericcode.Theentrynumberiscomprisedofthethree digitfilercode,followedbythesevendigitentrynumber,andcompletedwiththeone digitcheckdigit. EntryFilerCoderepresentsthethree-characteralphanumericfilercodeassignedtothe filerorimporterbyCBP. EntryNumberrepresen...
BrDU assays were conducted using the Cell Proliferation ELISA, BrDU-Kit (Merck, Darmstadt, Germany) according to the manufacturer’s instructions. Crystal violet staining was performed as described previously10. Clonogenicity assays For colony- and sphere-formation assays, 2500 cells/ml were seeded ...
For ChIP-sequencing, DNA sequencing libraries were prepared from chromatin immunoprecipitated DNA using a library preparation kit (E6000s, New England Biolabs) according to the manufacturer’s instructions and sequenced using an Illumina Genome Analyzer. Tissue from untreated SARD1-HA transgenic plants wa...