CBP was cloned in two parts using single copy Xba1 site at position 3294-3299; CBP1-3299 was cloned into BamHI/XbaI sites in pFastBac HTA using primers (Fwd: DB_PCR_1; Rvs: DB_PCR_2); CBP3294-7326 was then cloned into XbaI/HindIII sites in the pFastBAC HTA-CBP1-3299 construct ...