The optimal amounts of cationic lipid reagent and DNA may change in the presence of serum; thus optimize conditions with serum if you plan to add it to the transfection medium. Most cells remain healthy for several hours in a serum-free medium. ...
Basic steps for performing cationic lipid transfection Step 1Perform dilutions Step 2Combine to form complexes Step 3Add complexes to cells Step 4Assay cells Dilute RNA, siRNA, or oligonucleotides and the transfection reag...
Insulin receptor activation and down-regulation by cationic lipid transfection reagents. BMC Cell Biol. 2004; 5 :7.Pramfalk C, Lanner J, Andersson M, Danielsson E, Kaiser C, Renström IM, Warolén M, James SR: Insulin receptor activation and down-regulation by cationic lipid transfection ...
(DOGS). Transfection reagentLipofectin, which is a 1:1 w/w mixture of the DOTMA and DOPE was a great commercial success 30 years ago. Other successful commercial products arelipofectamineand lipofectamine 2000. The former is a 3:1 ratio of lipid DOSPA and theneutral lipid1,2-Dioleoyl-sn-...
Complexes of the PE-encoding plasmid together with cationic lipid were injected into tumor xenografts in athymic nude mice. The tumor growth of transfected tumors was attenuated compared with control untreated tumors or tumors transfected with a nontoxin-expressing vector. These results indicate the ...
SM-102 is the only ionizable amino cationic lipid currently clinically approved for RNA therapeutics and can be used to synthesize lipid nanoparticles (LNPs). It shows potential in the development of LNPs for the delivery of mRNA-based vaccines. ...
Fixed cationic lipids have been proven to be good transfection agents in liposomes and lipid nanoparticles. Generally considered non-toxic at lower concentrations, these lipids do present some toxicity concerns when used in higher concentrations
Attempts should be made to improve the transfection efficiency of lipid/pDNA complexes into human islets. Conjugation of any targeting ligands to cationic gene carriers will not be very useful, since we transfect human islets ex vivo. Moreover, the net positive charge of LipofectAMINE/pDNA complex...
The present invention provides a compound represented by the formula (Ia) as a novel cationic lipid that forms a lipid particle and also provides a lipid particle comprising the compound. The present invention further provides a nucleic acid lipid particle containing the lipid particle, and a pharm...
Moreover, the transfection efficiency of this new cationic lipid reagent remained high in serum-containing medium and under serum-free conditions. Furthermore, in vivo transfection with O-Chol showed high levels of gene expression specific to peritoneal tumor cells. Consequently, the O-Chol:DNA ...