Cell toxicity Inconvenience In contrast, lipid-mediated transfection: Yields high and previously unattainable transfection efficiencies Works in a wide variety of eukaryotic cells Is simple to perform Ensures consistently reproducible results The main drawback of c...
Cell toxicity Inconvenience In contrast, lipid-mediated transfection: Yields high and previously unattainable transfection efficiencies Works in a wide variety of eukaryotic cells Is simple to perform Ensures consistently reproducib...
We evaluated liposome concentration/toxicity relationships in an in vivo rat lung transplantation model.Left lungs were harvested and infused via the pulmonary artery with chloramphenicol acetyltransferase (CAT)-DNA/lipid 67 (cationic lipid)/dioleoylphosphatidylethanolamine...
The type of linker group between head group and lipid chain determine the biodegradability of the lipid. The ester bond present in DOTAP makes it biodegradable and reduces toxicity hence is more suitable for in-vivo gene delivery compared to DOTMA. Cationic liposome consisting of N′,N′-dioctade...
Two novel cationic lipids based on protonated cyclen and steroid (cholesterol or diosgenin) moieties with carbamate linkage have been designed and synthesized for gene delivery. Cationic liposomes were easily prepared from each of these lipids individually or from the mixtures of each cationic lipid ...
Sengupta,S.,Tyagi,P.,Chandra,Kochupillai,V.,Gupta,K.Encapsulation in cationic liposomes enhances antitumour efficacy and reduces the toxicity of etoposide, a topo-isomerase II inhibitor. Pharmacology . 2001Patlolla RR and Vobalaboina V 2008 Folate-targeted etoposide-encapsulated lipid nanospheres. ...
61 A large variety of cells were assessed in this report (human, mouse, rat, Xenopus, and Drosophila cells), and the limitations associated with the toxicity of the DOTMA/DOPE agent, together with a needed optimization of the mRNA sequence for improved translation, have been addressed. Some ...
membrane and allows the introduction of RNA to the cell due to the inability to distinguish the liposome from the cell membrane. Advantages of cationic lipid-mediated transfection include high efficiency, ability to transfect a wide range of cell types, reproducibility, low toxicity, and simplicity....
The lipid has low toxicity and is biodegradable.Data Hygroscopic NoLight Sensitive NoMolecular Formula C42H85NO8PClPercent Composition C 63.17%, H 10.73%, Cl 4.44%, N 1.75%, O 16.03%, P 3.88%Purity >99%Stability 1 YearsStorage Temperature -20°C...
The nonliposomal FuGENE 6 and the cationic liposomal Lipofectamine 2000 are examples of reagents that can successfully transfect most adherent and suspension cell types (including several primary and hard-to-transfect cell types) with negligible toxicity and a minimal number of manipulations. Importantly...