In addition to using NaPP1 to inhibit PKCe in these assays, we also confirmed the specificity of the PKCe siRNA by recovering the phenotype with an siRNA-resistant PKCe construct. In this system, the metaphase delay is recovered as shown by a decrease in the metaphase transition speed of 56...
(48), data shown are from three independent experiments. The biotinylated, tailed, nicked DNA was attached to M280 beads using the Kilobase Binder Kit (Invitrogen). Show abstract The MukB–topoisomerase IV interaction is required for proper chromosome compaction 2017, Journal of Biological Chemistry...
We also demonstrate that the production of catenanes and knots is influenced by two factors: (1) supercoiling in the DNA substrate, supercoiled DNA substrates yield significantly more catenated and knotted products than nicked circular substrates; and (2) mutations in the loxP site, a class of ...