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Library preparation strategies were tailored to the sequencing technologies used in this study. Libraries preparation and genome sequencing Genomic DNA (gDNA) sequencing data were generated using multiple platforms, including Oxford Nanopore (ONT) long reads, PacBio high-fidelity (HiFi) reads, Illumina ...
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DNA (50 μg) was sub- mitted to the Institute for Genome Sciences (University of Maryland) for size selection and preparation of se- quencing libraries. Libraries were sequenced on 12 SMRT cells of a PacBio Sequel (Pacific Biosciences; Menlo Park, CA), generating 7,239,750 reads (46.7 ...
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