利用PyMOL软件对蛋白质进行去水、去磷酸根等操作,保存为PDB文件。采用Molecular Operating Environment 2019软件对化合物进行能量最小化,对靶点蛋白进行预处理并寻找活性口袋,进行半柔性分子对接,根据结合能大小评估二者的结合活性,并将结果通...
在线粒体膜电位较高时,JC-1聚集在线粒体的基质中形成聚合物产生红色荧光;在线粒体膜电位较低时,JC-1不能聚集在线粒体的基质中,此时JC-1为单体,可以产生绿色荧光。 2.6分子对接预测白屈菜碱、延胡索乙素和阿霉素与细胞焦亡相关...
caspase-11 (C254A)-p22/p10 结构(PDB ID 代码 6KMU)用作小鼠 CASP11 (p22/p10) 模型。基于全长鼠 GSDMD(PDB ID 代码 6N9N)的结构,程序 Modeler 生成了带有切割接头的全长 mGSDMD 中鼠 GSDMD-N 片段的失活构象(mGSDMD-Ni)。此外,基于小鼠 GSDMA3(PDB ID 代码 6CB8)和人类 GSDMD(PDB ID 代码 6...
IL-1β activity must be fine-tuned to enable anti-microbial responses whilst limiting collateral damage. Here, we show that precursor IL-1β is rapidly turned over by the proteasome and this correlates with its
The PDB format of NLRP3 (ID: 6npy) [14] was downloaded from RCSB (https://www.rcsb.org/), waters and het groups were deleted, and hydrogens were added, and then the docking procedure was submitted [15]. 2.2. Animals Male Sprague-Dawley (SD) rats were purchased from Pizhou Oriental...
A caspase catalytic cleft. The active site cleft of caspase-2 bound to the peptapeptide inhibitor acetyl-Leu-Asp-Glu-Ser-Asp-aldehyde (Ac-LDESD-CHO), taken from PDB code 1PYO.68The caspase is shown as a translucent electrostatic potential surface where blue hues denote positive potential and...
Immunogen This antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to SQSTM1. Gene ID 8878 SwissProt Q13501 Synonyms OSIL antibody; Oxidative stress induced like antibody p60 antibody p62 antibody p62B antibody Paget disease of bone 3 antibody PDB 3 antibo...
BID structure (pdb 1DDB) was modeled in SwissPDBViewer (Swiss Institute of Bioinformatics, Lausanne, Switzerland) and the resulting model was edited with Pymol (Delano Scientific, San Carlos, CA, USA), as previously described.14 Single cell gel electrophoresis (COMET) Neutral comet assay was pe...
利用胱天蛋白酶-1(PDB 1IBC)、胱天蛋白酶-4(PDB 6KMZ)和胱天蛋白酶-11(PDB 6KMV)生成该比对。 图10显示了使用CJ11检测胱天蛋白酶底物的蛋白质印迹。切割受TRAIL刺激的细胞(+)并且进行免疫沉淀和随后的蛋白质印迹。 具体实施方式 I.定义 如本文所用的“疏水性氨基酸”意指色氨酸、苯丙氨酸、酪氨酸、...
Human native SERPINB1 structure; PDB 4GA7. The CBM of SERPINB1 consists of β-strands (s1C, s4B and s5B) depicted in red color. The surrounding α-helices (αH, αG and αA) are colored in light cyan and β-strands (s3B, s2B, s1B and s6B) are shown in dark gray. The RCL...