进行caspase-1活化检测时，需要考虑细胞类型的影响，不同细胞对caspase-1活性调节存在差异。巨噬细胞中NLRP3诱导的caspase-1活性快速发生，导致GSDMD和pro-IL-1β迅速裂解，随后活性消失，细胞焦亡。中性粒细胞则表现出较弱且长效的caspase-1活性。检测过程中，细胞裂解液和培养基中的p20和p10蛋白含量存在...
After blocking with 5% skimmed milk or Odyssey blocking buffer (LI-COR Biosciences) for 1 h at room temperature, the membranes were probed with the following primary Abs: mCaspase-1p20 (AdipoGen, clone Casper-1), mCaspase-1p10 (AdipoGen, clone Casper-2), mIL-1β (Cell Signaling ...
上海易佰聚经贸有限公司所提供的caspase-9 p10 (m)-PR质量可靠、规格齐全,上海易佰聚经贸有限公司不仅具有专业的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息!
别名Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage...
caspase-7 p10 (E-11): m-IgG1BP-HRP 套装 sc-545310 200 µg Ab; 20 µg BP $354.00 caspase-7 p10 (E-11) 中和勝肽 sc-271747 P 100 µg/0.5 ml $68.00 推荐相关产品 單擊選擇的應用程序按鈕 WB IF 您可能也感兴趣 caspase-7 p20 抗体 (C-12) ...
cas pase的结构可分为前结构域(p r odomain)、大亚基和小亚基[后二者统称为催化域(catalytic domain)][1]三部分。在p r ocas pase被切割为大、小两个片段(约20k Da和10k Da,分别为p20和p10)及一个N-端前区后,两大两小共4个片段聚合成为含有两个位点活化了的cas pase。1 cas pase-8参与细胞凋亡的...
-20。C避光 使用说明书 1份 自备材料: 1、水浴锅或恒温箱 2、96孔板 3、酶标仪或分光光度 操作步骤(仅供参考): 1、制备标准曲线: ①按照Caspase Lysis buffer:Assay buffer=1:9的比例配制适量的标准品稀释液。 ②用标准品稀释液稀释pNA(10mM),使pNA分别达到200μM、100μM、50μM、25μM、12.5μM、6.2...
Results are expressed as the mean of three separate brains±S.E.M. Z-VEID and Z-IETD treatment significantly reduced the amount of activated caspase-6 (p10), caspase-8 (p18), and caspase-3 (p17) at 48 h following thromboembolic ischemic injury; (h and i) Caspase activity assay ...
GOUGEON, M. L.; KROEMER; G. Charming to death: caspase-dependent or -independent? Cell Death and Differentiation, v. 10, n. 3,p. 390-392, 2003.Gougeon, M.L.; Kroemer, G. Charming to death: Caspase-dependent or -independent? Cell Death Differ. 2003, 10, 390-392....
values of the β core in the pro-caspase-8-FLIPLand the caspase-8-FLIPL model were calculated using the β core of caspase-8-caspase-8 as a template (PDB file 1F9E (Ref. 45)). As a negative control, the r.m.s.d. of the β core in a caspase-3-FLIPL model was also ...