caspase-1 Antibody (A-19) has been replaced by a more specific monoclonal antibody, caspase-1 (D-3) that gives a stronger signal & more reproducible data.
caspase-1 p20 抗体(D-4)是一种 IgG2b κ 小鼠单克隆 caspase-1 p20 抗体,可通过 WB、IP、IF、IHC(P) 和 ELISA 检测到小鼠和大鼠来源的 caspase-1 前体和 p20 亚基。caspase-1 p20 抗体(D-4)既可以是非偶联抗 caspase-1 p20 抗体形式,也可以是多种偶联形式的抗 caspase-1 p20 抗体,包括琼脂糖、HRP...
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(starting dilution 1:50, dilution range 1:50-1:500) and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500). Suitable for use as control antibody for caspase-1 siRNA (h): sc-29235, caspase-1 siRNA (m): sc-29922, caspase-1 ...
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sc-514) antibodies were purchased from Santa Cruz Biotechnology, Inc. Anti-GAPDH antibody (1:1000 dilution; Ab9485) was obtained from Abcam. Anti-IL-1β antibody (1:1000 dilution; catalog no. 2022) was purchased from Cell Signaling Technology. Human recombinant caspase-1 was from Merck. ...
secondary antibody (Abgent, 1:1000) dissolved in the blocking buffer for 2 h at RT. After incubation with secondary antibodies, cells were washed and counterstained with DAPI solution (1 μg/ml in the blocking buffer) for 10 min at RT. Cells were analyzed by fluorescence microscope (Leica,...
Immunoprecipitation was performed as previously described [12,17]. Cells were solubilized in lysis buffer. The precleared lysates were incubated with the corresponding antibody (about 1–1.5 μg each) in the presence of 20 μl of Protein A/G Agarose (Pierce) overnight with constant agitatio...
Primary antibodies including; anti caspase-1 antibody]EPR19672[ (catalog number#ab207802, Abcam, MA,USA), anti NLRP3antibody [EPR23094–1] (catalog number#ab263899, Abcam, MA,USA, and anti NF-κB p65 (F-6) antibody (catalog number#SC-8008, Santa Cruz Biotechnology., TX, USA). ELISA ...
The membrane was primary probed overnight with the following anti-sera at 4 °C: anti-mouse IL-1β antibody (AF-401-NA, R&D Systems, Minneapolis, MN, USA), anti-Caspase-1(p20) antibody (AG-20B-0042-C100, AdipoGen Co., San Diego, CA, USA), anti-Asc antibody (sc-22514, Santa ...