Figure 3. Fluorescence Flow Cytometry analysis of CaspACETM FITC-VAD- FMK In Situ Marker-labeled apoptotic Jurkat cells. Human Jurkat cells were treated with 100ng/ml anti-Fas antibody for 4 hours, or left untreated, prior toincubation with 10碌M CaspACETM FITC-VAD-FMK In Situ Marker. ...
CaspACE™ FITC-VAD-FMK In Situ Marker(CaspACE™ FITC-VAD-FMK 原位标记物) 是一种泛caspase 的抑制剂Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[Omethyl]-fluoromethylketone) 的荧光类似物。氟硫氰酸荧光素(FITC) 基团取代了N- 端的卞酯基(Z),从而成为凋亡的荧光标记物。这种结构使该抑制剂进...
Figure 3. Fluorescence Flow Cytometry analysis of CaspACETM FITC-VAD- FMK In Situ Marker-labeled apoptotic Jurkat cells. Human Jurkat cells were treated with 100ng/ml anti-Fas antibody for 4 hours, or left untreated, prior toincubation with 10µM CaspACETM FITC-VAD-FMK In Situ Marker. ...
CaspACE FITC-VAD-FMK In Situ Marker as a Probe for Flow Cytometry Detection of Apoptotic CellsINTRODUCTIONSeveral pathways lead to apoptotic cell death through the cleavage ofzymogens and the generation of specific proteases called caspases. The caspase family c...