1Datasheet for H1975/Cas9-hyg Stable Cell Line Catalog number:SL529Product:H1975cell line stably expressing CRISPR Cas9nuclease Description:This product is a cell line stably expressing the CRISPR Cas9nuclease.This cell line also expresses the hygromycin resistance gene.In combination with separately ...
Horizon’s Cas9 and dCas9-VPR stable cell lines were generated using its Edit-R Lentiviral particles with a blasticidin resistance cassette and are provided in pooled format. The cell lines are QC verified and validated to ensure stable expression and functionality of Cas9 or dCas9-VPR en...
【神助攻系列】Cas9稳定表达细胞系 【神助攻系列】Cas9稳定表达细胞系 CRISPR-Cas9 CRISPR/Cas9基因敲除系统由有DNA切割活性Cas9蛋⽩ 蛋⽩及识别特异靶点的gRNA组成，由于只需对系统中的gRNA进⾏编辑就能实现靶点识别，极⼤了简化了构建基因敲除载体的⼯作。CRISPR-Cas9 赋予我们精准编辑基因的能⼒ 在 Google...
Stable cell line validationgRNA designCRISPR, Clustered Regularly Interspaced Short Palindromic Repeat, as a powerful genome engineering system has been widely accepted and employed in gene editing of a vast range of cell types. Comparing to zinc finger nucleases (ZFNs) or transcription-activator-like...
Invitrogen LentiArray Cas9 Lentivirus provides an efficient method to drive stable Cas9 nuclease expression in a wide variety of cell types, including hard-to-transfect cells. The LentiArray Cas9 Lentivirus construct includes a human codon-optimized Cas9 with two n...
If a single site of the genome is to be targeted regularly, this requirement for transfection could be avoided by creating a stable cell line expressing Cas9 and the required gRNA, greatly simplifying the method. The speed of making recombinant VACVs by this method is noteworthy because it ...
generated the dCas9 stable cell line; P.D.H., A.E.T. and S.K. purified Cas9; P.D.H. contributed to in vitro binding assay; S.C. contributed to ChIP experiments with transient transfection. X.W., F.Z. and P.A.S. wrote the manuscript with help from all other authors. R.J....
Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695 Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. ...
Validated protocols for a large number of cell types help you achieve success faster TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex wi...
We next tested whether an extra chromosome in aneuploid cells could be eliminated by CRISPR/Cas9 editing. We focused on an ES cell line with an extra human chromosome 14 (hChr14), which was established by chromosome transfer (Fig.7a, b; see “Methods”) and known to be stable in cell ...