explosive growth has been recorded in the study of CRISPR technology, with tens of thousands of CRISPR-related articles published. In October 2020, the Nobel Prize in Chemistry was awarded to French microbiologist Emmanuelle Charpentier and American biologist Jennifer Doudna for “developing...
CRISPR/Cas9系统由一个单链向导RNA（small guide RNA, sgRNA）和一个具有核酸内切酶功能的Cas9 蛋白组成...
fusion with proteins to add necessary functions, e.g., control of DNA repair pathways (Richardson et al.2023), fusion with enzymes to create novel genome editors, like prime editors or base editors (Tao et al.2023; Saber Sichani et al.2023; Porto...
单碱基编辑器有2种,分别为2016年、2017年发展起来的技术胞嘧啶碱基器(cytosine base editors, CBEs)和腺嘌呤碱基编辑器(adenine base editors, ABEs)。CBEs系统的组成包括:具有单链切割活性的切口Cas9 (nicking Cas9, nCas9)蛋白、sgRNA、胞嘧啶脱氨酶(cytosi...
Crowther MD, Dolton G, Legut M, et al. Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class Ⅰ-related protein MR1. Nat Immunol, 2020, 21: 178-185.[8] He XY, Ren XH, Peng Y, et al. Aptamer/ peptide-functionalized genome-editing ...
The CRISPR-Cas9 system has frequently been used for genome editing in Streptomyces; however, cytotoxicity, caused by off-target cleavage, limits its application. In this study, we implement innovative modification to Cas9, strategically addressing challe
Finally, we demonstrate that SpRYc can be integrated within base editing architectures to edit disease-related loci for potential therapeutic purposes. While SpRYc serves as a step forward towards unrestricted, fully programmable genome editing, its development, more importantly, represents a culmination...
近日,河北农业大学赵建军/李君团队在aBIOTECH上在线发表了题为“Expanding the targeting scope of CRISPR/Cas9-mediated genome editing by Cas9 variants in Brassica”的研究论文,显著提高CRISPR/Cas9技术在芸薹属蔬菜中的编辑能力。 本研究首...
Therapeutic Strategies of Cas9 nuclease, base editor and prime editor. Different Cas9-related editing tools (above) can produce different types of edits to the genome (middle) and thus can cause different changes to the corresponding genes (below) ...
The outcome of CRISPR-Cas-mediated genome modifications is dependent on DNA double-strand break (DSB) processing and repair pathway choice. Homology-directed repair (HDR) of protein-blocked DSBs requires DNA end resection that is initiated by the endonuc