技术服务 Cas9-Cell line Gene Mutation service(CGM)基因点突变细胞系是细胞基因研究方法中一个重要手段,它可以在细胞内原位对点突变的功能进行研究。在细胞系上实现高效的转染是实现基因点突变的前提;提高效率要解决问题是:Cas9切割位点与“点突变”位点之间距离导致点“点突变”效率大幅下降的问题,还有“点
Cas9-Cell line Gene Mutation service(CGM)基因点突变细胞系是细胞基因研究方法中非常有用的一个手段,可以在细胞内原位对点突变的功能进行研究。在细胞系上实现高效的转染是实现基因点突变的前提;提高效率要解决问题是:Cas9切割位点与“点突变”位点之间距离导致点“点突变”效率大幅下降的问题,还有“点突变”载体的...
We used recombinase-mediated cassette exchange to replace the3xP3::GFPtranscription unit with adsxFCRISPRhgene drive construct that comprised an RFP marker gene, a transcription unit to express the guide RNA (gRNA) targetingdsxF, andcas9under the control of the germline promoter ofzero population ...
Hacein-Bey-Abina, S. et al.LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1.Science302, 415–419 (2003).
When using the CRISPR-Cas9 system to knock out gene expression or knock in a specific mutation, the design, production, and delivery of high-quality guide RNAs (gRNAs) are critical to success. Whether you need transfection-ready gRNAs to use with TrueCut Cas9 Proteins or you need to harness...
When using the CRISPR-Cas9 system to knock out gene expression or knock in a specific mutation, the design, production, and delivery of high-quality guide RNAs (gRNAs) are critical to success. Whether you need transfection-ready gRNAs to use with TrueCut Cas9 Proteins or you need to harness...
Thus far, we have shown that targeted chromosome loss can occur when using Cas9 to disrupt a desired gene, which predominantly occurs through end-joining DNA repair pathways. Tremendous effort has also been invested toward using Cas9 genome editing to correct a mutation or insert a gene by homol...
or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted gene. The ideal end result is a loss-of-function mutation within the targeted gene. However, the strength of the knockout phenotype for a given mutant cell must be validated experimental...
gene expression, although finally, the expression did not differ significantly between WT and PUB7-GE or between individuals. This suggested that althoughOsPUB7harbored a mutation introduced using the CRISPR/Cas9 technology, the transcriptional regulatory region of the gene functioned normally. ...
EDITGENE provides various cell lines gene editing services and related products, such as CRISPR library screening, knockout cell line, Knock in cell line, point mutation cell line Cas12a, etc.