近日,电子科技大学张勇教授联合马里兰大学Yiping Qi博士在Plant Communications在线发表了题为“Hypercompact CRISPR-Cas12j2 (CasΦ) enables genome editing, gene activation, and epigenome editing in plants”论文。该文基于V-J型CRISPR-Cas基因簇成员CRISPR-Cas12j2核酸酶系统,测试了其在植物基因组敲除编辑中的可行...
One significant obstacle in this arena is the substantial size of Cas proteins, such as SpCas9, which is approximately 190kDa, complicating their delivery, particularly via viral vectors. To overcome this challenge, our research introduces the hypercompact Cas12j2 system, a groundbreaking development...
Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells. Gene/Insert name CasPhi-2 Alt name Cas12j2 Tag / Fusion Protein 2xSV40NLS-2xFLAG (C terminal on insert) 参考文献 CRISPR-CasPhi from huge phages is a hypercompact genome editor...
近日,Plant Communications在线发表了电子科技大学张勇教授、马里兰大学Yiping Qi博士课题组合作完成的:“Hypercompact CRISPR-Cas12j2 (CasΦ) enables genome editing, gene activation, and epigenome editing in plants”论文。该文基于V-J型CRISPR-Cas基因簇成员CRISPR-Cas12j2核酸酶系统,测试了其在植物基因组敲除编...