Without a standard material, the industry loses its potential for scale-up and commercialization. 1-Material Inc. sees the need and the necessity to standardize Organic Nano Electronic (ONE=1) materials for organic thin film applications and also understands the challenges to do so. With its accu...
[0201]37℃下使用T7 RiboMAX快速大量RNA制备试剂盒(Express Large Scale RNA Production kit)(Promega)从大约1μg HindIII线性化的crRNA转录质粒中体外转录Cas12a crRNA16小时。通过添加1μL RQ1 DNase在37℃下降解DNA模板15分钟,随后用miRNeasy Mini试剂盒(Qiagen)纯化RNA。体外切割反应由裂解缓冲液(10mM Hepes ...
24.Gagnon,J.A.等人Efficient mutagenesis by Cas9 protein-mediatedoligonucleotide insertion and large-scale assessment of single-guide RNAs[通过Cas9蛋白介导的寡核苷酸插入进行的有效诱变和单指导RNA的大规模评估].PLoS One[公共科学图书馆·综合]9,e98186(2014)。 其他实施例 应理解,虽然已经结合其详细描述,对本...
Differential linearity error Total error : : The deviation between the actual conversion characteristics and a line that joins the zero-transition line ( “00 0000 0000” “00 0000 0001” ) to the full-scale transition line ( “11 1111 1110” “11 1111 1111” ) . ...
Without a standard material, the industry loses its potential for scale-up and commercialization. 1-Material Inc. sees the need and the necessity to standardize Organic Nano Electronic (ONE=1) materials for organic thin film applications and also understands the challenges to do so. With its accu...
Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Science 2014, 343, 84–87. [CrossRef] [PubMed] 11. Koike-Yusa, H.; Li, Y.; Tan, E.P.; Velasco-Herrera, M.D.C.; Yusa, K. Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA ...