LUT1 (beta-carotene hydroxylase) is a heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Catalytic activity is specific for epsilon- and beta-ring hydroxylation of alpha-carotene. Alternative names: Cytochrome P450 97C1, Carotene epsilon-monooxygenase, chloroplastic. ...
摘要: Nucleic acid sequences coding for a protein having ,-carotene 15,15-monooxygenase activity and their uses in diagnostics, the synthesis of vitamin A and methods for the introduction of the ,-carotene 15,15-monooxygenase cDNA into host cells are disclosed....
β-carotene 15,15'-monooxygenase (BCMO1) catalyzes the crucial first step in vitamin A biosynthesis in animals. We wished to explore the possibility that a carbocation intermediate is formed during the cleavage reaction of BCMO1, as is seen for many isoprenoid biosynthesis enzymes, and to determi...