Binding of KIF1A to fully tyrosinated and detyrosinated microtubules was performed as described above, but using 20 nM TRITC-labeled fully tyrosinated and 20 nM Alexa Fluor 647-labeled fully detyrosinated GMPCPP-stabilized microtubules, and 25 nM Oregon Green-labeled KIF1A(1-393)-GCN4-Halo...