Remodeling and labellingE. coliPG To visualize PG, cells were grown in an optimized amount of either NAM derivatives1–3. After incubation (0.2% (w/v) of NAM derivative, see Methods andSupplementary Figs 1a and 2), a subsequent Copper (I)-catalysed azide-alkyne cycloaddition, also known as...
aReactions catalyzed by glycoside hydrolases (GHs), glycoside phosphorylases (GPs) and glycosyltransferases (GTs) in Glycoside Hydrolase family 65 (GH65).bReaction scheme for the inverting single displacement mechanism of GH65 enzymes. For GHs: ROH = a carbohydrate derivative and R’OH =...
(the chemistry was developed in parallel with a threonine derivative not shown here) provided glycoconjugate23. The azide was converted to acetamido compound24in two steps in nearly quantitative yield. A mild deprotection of the acetates provided glycoconjugate25. This general approach has been ...
Catabolic flexibility, a bacterial physiological status under which a wider range of substrates can be utilized [46], is often induced in cells with a reduced growth rate [45]. Furthermore, growth rate reduction has been shown in different bacterial species to govern their resistance to different...