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货号: YZ-0470 品牌: Extrasynthese 暂无详情 产品简介 CAS1260-17-9 分子式C22H20O13 分子量492.390015 储存条件Store in dry and dark place. 单位瓶 货期4周 规格100mg 联系我们 电话:021-50837765 手机:15221999938 邮箱:sales@jinpanbio.com 联系我们 ...
Then the secondary mouse anti-rat IgG antibody (Table S8), also diluted in Perm/Wash buffer, was applied. Cells were subsequently washed and analyzed. LCLs were generated by infection of PBMCs or CBMCs with EBV/M81/fLuc at a MOI of 0.1 overnight. The next day, cells were centrifuged ...
followed by a single wash and addition of Fc block (BioLegend #101320), then antibody staining. Spleens were macerated over a 70 um cell strainer using the piston from a 3 ml syringe to create single cell suspensions, after which 1-2e6 cells were transferred to flow cytometry tubes ...
When the tumor engraftment grew up to 100~2000 mm3 measured by a caliper, xenograft tumor tissue was mechanically dissociated by the flat end of a plunger after harvesting and passed through 100 μm cell strainer followed by 2 wash steps with PBS to obtain a homogenous single cell ...