Gene synthesis in combination with an optimization algorithm for codon usage in humans (ATUM, Newark, CA) was used to design genes of interest.E. coliDH5alpha were used for cloning and plasmid generation. Plasmids were purified using DNA isolation kits (Qiagen). ...
For staining with FITC-labeled human CD19 (20-291) Protein (AcroBiosystems, Newark, DE, USA), 5 μL of antibody were added in 0.1 mL of wash buffer and incubated at 4 °C for 45 min in the dark. For staining with the goat F(ab’)2 anti-human IgG (H+L)-RPE (Jackson Immuno ...