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The affinity of KB cells for the mediator was verified by incubation of KB cells with 10 nM mediator for 1 h at room temperature, followed by staining with an anti-FITC antibody conjugated to a PE dye (Abcam, Cambridge, UK) for more than 45 min. After being washed twice, the cells ...
CAR T-cell therapy as a part of adoptive cell therapy (ACT), has become one of the most rapidly growing and promising fields in the Immuno-oncology. As compared to the conventional cancer therapies, CAR T-cell therapy is the single-dose solution for the treatment of various cancers, signifi...
followed by a single wash and addition of Fc block (BioLegend #101320), then antibody staining. Spleens were macerated over a 70 um cell strainer using the piston from a 3 ml syringe to create single cell suspensions, after which 1-2e6 cells were transferred to flow cytometry tubes ...
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whereas truncated DcUGT2 protein missing only the transmembrane domain/cytoplasmic tail retained activity. The yeast-produced ΔSP-DcUGT2ΔMD-Strep protein remained associated with the membrane protein fraction, indicating that it had been targeted to the ER or associated with other cellular membrane st...
然而,在替代性实施方案中,可利用其它已知抗CD74抗体,诸如LS-B1963、LS-B2594、LS-B1859、LS-B2598、LS-05525、LS-C44929等(LSBio,Seattle,Wash.);LN2(San Diego,Calif.);PIN.1、SPM523、LN3、CerCLIP.1(Cambridge,Mass.);At14/19、Bu45(Raleigh,N.C.);1D1(Taipei City,Taiwan);5-329(San ...