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Lentiviral vector integration site analysis further revealed a high abundance of a clone with monoallelic integration at the downstream (nearly 14 kbp away) of ETS1 gene without affecting its transcription confirmed by quantitative PCR (Fig. 6c, Supplementary Fig. 7), ruling out the association ...
Sequences were synthesized (Bio Basic, Markham, Ontario, Canada) and sub-cloned into the MSGV Hu Acceptor retroviral vector (Addgene #64269) after removal of human T-cell receptor (TCR) genes. Phoenix-GP cells were co-transfected with the HER2-CAR vector and pCMV-VSV-G (Addgene #8454...
Frequency of tumour-infiltrating CD8+CAR T cells expressing IFNγ and TNF. Top, combined samples. Bottom, data from individual mice.b, Frequency of CAR T cells expressing 0, 1, 2 or 3 effector proteins (IFNγ, TNF or GZMB).c, MC38-HER2 tumour growth in mice treated as in Fig.1. ...
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The mouse CAR19 vector was kindly provided by Dr. M. Sadelain (Memorial Sloan-Kettering Cancer Center, New York, NY). The CAR sequence is based on the immunoglobulin heavy and light chain sequences of anti-mouse CD19 antibody clone 1D3, followed by the mouse CD8 transmembrane region, mouse...
After 48 h, activated T cells were transduced with the CD19/CD20 lentivirus vector. After CAR gene transduction, the cells were cultured outside the body with the addition of IL-2 three times a week until the desired number of cells was reached. The activated beads were magnetically ...