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Primary T cell cultures and T cell-related assays were performed in Roswell Park Memorial Institute (RPMI) 1640 (PAN Biotech), supplemented with 10% FBS (PAN Biotech), 100 U/mL penicillin, 100 µg/mL streptomycin, 0.05 mM β-mercaptoethanol (Sigma-Aldrich, Darmstadt, Germany), 20 mM 4-...