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The cells were cultured at 1 × 106 cells/mL in RPMI-1640 medium (PanEco, Russia) containing L-glutamine (PanEco, Russia), penicillin/streptomycin (PanEco, Russia), and 10% fetal bovine serum (FBS) (Hyclone, USA). Dynabeads Human T-activator CD3/CD28 (Invitrogen, Carlsbad, CA, USA) ...