Afterwards, the cells were cultured in StemSpanTM medium (STEMCELL Technologies GmbH, Cologne, Germany), containing 2% FCS, 100 μg/mL penicillin/streptomycin, 2 mM N-acetyl-L-alanyl-L-glutamine, and supplemented with 10 ng/mL of fms-related tyrosine kinase 3 ligand (FLT3-L), 10 ng/mL ...