2d). Clinical improvement in spinal cord function was not observed after the second infusion as it was after the first (Supplementary Table 4). Both spinal and brain disease progressed further by 2 months following i.c.v. infusion and she received no further GD2-CAR T cell infusions. ...
When endogenous CD28 was co-activated by CD80/86, tumor necrosis factor (TNF) and interferon γ (IFNγ) secretion of disLCK-CD137-CAR-T cells was restored (Figure 2D). LCK-deficient CAR1-Jcam and TCR-Jcam cells also showed restored IL-2 production and downstream signaling when CD28 co-...
2d). Clearly, the C-terminal domain specifically interacts directly with methylated NFIB (Fig. 2f). Using a co-IP assay, we also show that endogenous NFIB and TRIM29 interact, and that this interaction is prevented by CARM1 inhibitors (Supplementary Fig. 2b). The C-terminal domain of TRIM...
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In contrast to results obtained with 3DHSP, HER2-CAR T cells did not kill HER2-expressing BT474 cells in 2D culture. We observed that while SKOV3 cells spread their bodies more uniformly across the well surface, BT474 cells formed compact clusters and spread their cell bodies to varying ...
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(MDRmidMGhi). This was also reflected by higher MDR and MG staining intensities, indicative of properly regulated membrane potential and mitochondrial mass36(Fig.2d,e). Furthermore, RNA-seq of in vitro-stimulated CAR T cells revealed that an oxidative phosphorylation signature was among the most...