1.1. Capillary Electrophoresis of Proteins Capillary electrophoresis (CE) is a separation technique that combines aspects of both gel electrophoresis and high performance liquid chromatography (HPLC). As is the case for gel electrophoresis, the separation in CE is based upon differential migration in ...
L., Capillary Electrophoresis of Proteins and Peptides, Humana Press, Totowa, New Jersey, 2004.Burgi D, Smith AJ (2001) Capillary electrophoresis of proteins and peptides. Curr Protoc Mol Biol 10:10.20.1–10.20.13Strege, M. A., Lagu, A. L., Capillary Electrophoresis of Pro- teins and...
CAPILLARY ZONE ELECTROPHORESISPARAPROTEINSSerum and urine protein electrophoresis is an essential component in the investigation and monitoring of patients with B cell malignancies. Automated capillary zone electrophoresis is sensitive and reliable and is comparable to agarose electrophoresis systems for the ...
历史 The Nobel Prize in Chemistry 1948 was awarded to Arne Wilhelm Kaurin Tiselius "for his research on electrophoresis and adsorption analysis, especially for his discoveries concerning the complex nature of the serum proteins[1]" 原理 毛细管电泳开始于20世纪60年代。在进行分析时将毛细管内充满了电解...
Numerous efforts have been made to separate proteins by capillary zone electrophoresis (CZE). The most common optimization techniques are changing the pH of the running buffer, coating the capillary surface with a hydrophilic polymer, or using additives in the sample solution. Surface coatings and so...
Fused-silica capillary columns having cationic surfactant moieties (CSM) covalently attached to the capillary inner walls were introduced for the separation of proteins by capillary zone electrophoresis (CZE). The CSM capillary coating proved to be useful in the separation of basic and acidic proteins...
Capillary electrophoresis of proteins in a quality control environment. A method for determining the purity of recombinant carboxypeptidase B utilizing CE-SDS has been developed and validated for use in a manufacturing quality ... DL Good,S Cummins-Bitz,RM Fields,... - 《Methods Mol Biol》 被引...
Capillary zone electrophoresis (CZE) is a technique successfully used for the separation of proteins, peptides, and nucleic acids. Other applications where CZE may be useful include analysis of inorganic anions and cations, such as those typically separated by ion chromatography. Small charged molecule...
摘要: Reports on determination of amino acid composition of proteins by capillary electrophoresis from the Briar Cliff College of Sioux City in Indiana. Introduction into amino acids; Methodology of experiments; Discussion of results.年份: 1994 ...
In addition to free solution separations such as capillary zone electrophoresis (CZE) (4),analyte separation of peptides and proteins can also be effected by isoelectric focusing (IEF) (5–8), isotachophoresis (9), molecular weight sieving (10), and micellar electrokinetic chromatography (MEKC)...