A functional taxonomy of tumor suppression in oncogenic KRAS-driven lung cancer. Cancer Discov. 11, 1754–1773 (2021). Article CAS Google Scholar Motorin, Y. & Marchand, V. Detection and analysis of RNA ribose 2′-O-methylations: challenges and solutions. Genes (Basel) 9, 642 (2018)....
The DNA template required for the in vitro transcription was synthesized by PCR, in which the DNA sequence coding for eGFP, Firefly luciferase (FLuc), Gaussia luciferase (GLuc) and Renilla luciferase (RLuc) were amplified from pMRNA vectors containing the respective sequence. After purification (Nu...
Moreover, the ability of shRNA-targeting DAP5 to sensitize human cancer cells treated with the anticancer drug taxol to apoptosis indicates that DAP5 has an excellent potential to serve as a target for anticancer therapy in the future. Experimental Procedures Cell Cultures, Synchronization Procedures...
Fig. 1. REDCap Project Initiation Workflow: Study-specific databases are created using data dictionaries provided by the research team. After an initial demonstration, research teams use a custom MS-Excel file template to provide project metadata. The informatics team uses this file to create a pr...
As there is often no room for comprehensive implementation details, this report is intended to provide a compact but more accessible template for similar future projects. We present the design of a surrounding spherical cap loudspeaker array, including a description of the reproduction environment, ...
(containing the UMI and cell barcode) to both ends of full-length cDNAs during reverse transcription and template switching, and further amplified the cDNAs of each single cell based on a modified Smart-seq2 protocol21. After tagmentation with Tn5 transposases, fragments containing the tags and ...
the HHpred server60 for the detection of homologous structures, and subsequent comparative modelling using Modeller26 (as integrated in the Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de)) with the RRM of human poly(A) adenylate-binding protein 1 (PDB code 4f02) as a template model....
(e.g. double-data entry options); (5) data attribution and audit capabilities; (6) protocol document storage and sharing; (7) central data storage and backups; (8) data export functions for common statistical packages; and (9) data import functions to facilitate bulk import of data from ...
Besides neurological diseases and organismal survival, cancer is also in the top three of overrepresented diseases and function categories. This is not surprising, as (i) we are working with cell lines derived from tumor material and (ii) AZA has been shown to have anti-cancer effects by ...
Expression and function of lysophosphatidic acid LPA1 receptor in prostate cancer cells. Endocrinology 147, 4883–92 (2006). 10. Castelino, F. V. et al. Amelioration of dermal fibrosis by genetic deletion or pharmacologic antagonism of lysophosphatidic acid receptor 1 in a mouse model of ...