(F) 3D co-culture of PDAC cells with fibroblast cells exhibit altered expression of collagen-1, TGF-β, MMPs, and fibronectins in ECM similar to pancreatic cancer-related desmoplasia. 3D bioprinted matrigel-based co-culture constructs utilizing droplet-based growth organoids formed a representative...
Pancreatic cancer cell growth was reduced in low pH conditions, but acidic pH stimulated both proliferation and colony formation of PC3 cells54, while LNCaP cells underwent apoptosis55. Although the effect of low pH in PCa cells was not similar to PDAC cells, it remains to be determined whether...
A large fraction of polyp and CRC cells exhibit a stem-like phenotype, and we define a continuum of epigenetic and transcriptional changes occurring in these stem-like cells as they progress from homeostasis to CRC. Advanced polyps contain increasing numbers of stem-like cells, regulatory T cells...
F: MDECs exhibit increased migration when exposed to oncosomes compared with vehicle. Left panel, the vesicles are isolated from the plasma of mice with MyrAkt1 tumors. Right panel, the vesicles are derived from the medium of LNCaP/MyrAkt1 cells. Ctrl, control; SV, shed vesicle. G: Tumor...
Exquisite regulation of telomere length is essential for the preservation of the lifetime function and self-renewal of stem cells. However, multiple oncogenic pathways converge on induction of telomere attrition or telomerase overexpression and these events can by themselves trigger malignant transformation...
Hanbing Song and colleagues [18] analysed cell states associated with tumourigenesis in PCa samples by single-cell omics. They performed a comprehensive cell-level analysis of epithelial cell subsets, stromal cells, and TME by scRNA-seq. It was found that ERG-tumour cells exhibit common heterogene...
As the first-line chemotherapeutics for endometrial cancer therapy, cisplatin and paclitaxel exhibit well-inhibition effect on cancer cell growth, however, chemoresistance is still a major obstacle in endometrial cancer therapy [6]. Therefore, it is urgent to find a biomarker of chemoresistance and ...
Cell death pathways are among the most deregulated in cancer cells and are considered as critical aspects in cancer development. Through decades, our knowledge of the mechanisms orchestrating programmed cellular death has increased substantially, attributed to the revolution of cutting-edge technologies. ...
NE cells are a normal component of both the developing and mature prostatic epithelium. They produce a variety of neurosecretory products that exhibit growth-promoting activities, including parathyroid hormone-related peptides, neurotensin, serotonin, calcitonin and bombesin-related peptides, which suggests...
The virus was diluted (1:15) and added to 2.5×105 cells in a 6 well plate containing 8 μg/ml of polybrene (Sigma). Plates were centrifuged for 15 min, 1178×g at RT. For selection of virally infected cells, 2 μg/ml of Puromycin or 5 μg/ml of Blastocidin was used 24 hr...