After 72 h of co-culture, SH-SY5Y-GFP cells were separated from native hADSCs, hADSCs-BFP or hADSCs-IL2 using a FACS Aria III (BD Biosciences, San Jose, CA, USA). After separation, 5 × 104 sorted SH-SY5Y cells were immediately stained to detect apoptosis and necrosis using an APC...
A comparison of forest structure of post-harvest and post-fire residual patches is required for developing forestry strategies that can maintain biological diversity after harvest. This study aims to compare the structural complexity of current post-harvest and post-fire residual patches and unburned ...
Binding of extracellular ligands also stabilizes the extended open conformation, which shows enhanced separation of the integrin intracellular tails that, in turn, transmits signals to the cytoplasm (outside-in signaling). Because the equilibria of conformational changes and ligand binding are ...
injury or assault to a close relative, death of a close relative, death of a family member or other relative, marital separation or relationship breakup, interpersonal problems, unemployment, unsuccessfully looking for work for more than one month, major financial crisis, legal problems, or property...