Total RNA was extracted from adultA. spiniferususing the Total RNA Extraction Kit (Vazyme, Nanjing, China), the purity and integrity were tested by 1% agarose gel electrophoresis, and stored at − 80 °C. A total reaction volume of 20 μL cDNA was obtained using the cDNA synthesis ...
While the splicing reaction proceeds nearly to completion under the circularization conditions we used, some triphosphorylated linear precursor RNA is also present. We therefore applied additional steps of purification to the unpurified splicing reactions and confirmed circRNA enrichment by gel electrophoresis...
melting curve was plotted and agarose gel electrophoresis was performed to examine the specificity of PCR products. Each reaction was run in triplicate. The primer sequences used for qPCR are shown in Table S3. GAPDH was used as an internal control for lncRNAs and mRNAs, whereas U6 was used ...
Stable epigenetic changes appear uncommon, suggesting that changes typically dissipate or are repaired. Changes that stably alter gene expression across generations presumably require particular conditions that are currently unknown. Here we report that
Cells with H3K27me3-rich regions knockout also show changes in phenotype associated with cell identity, and altered xenograft tumor growth. Finally, we observe that H3K27me3-rich regions-associated genes and long-range chromatin interactions are susceptible to H3K27me3 depletion. Our results ...
Although repeat sequences constitute about 37% of carnivore genomes, the characteristics and distribution of repeat sequences among carnivore genomes have not been fully investigated. Based on the updated Repbase library, we re-annotated transposable ele
In the present study, we identified a novel NSCLC-associated lncRNA, namely, AL139294, which has not been reported in cancers or other diseases. AL139294.1 was found to be dramatically upregulated in the serum EVs of patients with NSCLC and could be transported by EVs to recipient cells. Tr...
To confirm the findings with POT1bΔCST, we employed shRNA-mediated knockdown of Stn1. Consistent with the results with the POT1bΔCST mutant, partial knockdown of Stn1 restored the ability of overexpressed POT1b to repress ATR signaling at telomeres. In contrast, knockdown of Stn1 did not...
Locus 3813 was matched to “Tetratricopeptide-like helical domain superfamily” proteins, which is a structural motif present in a wide range of proteins. In this case, it was found associated with “Pentatricopeptide repeat”, which is involved in multiple aspects of RNA metabolism [36]. ...
Fig 2.Northern blot analysis of culturedNK cells.SCIDspleen cellswere cultured with rhlL-2for 7 to 10 days. RNA was prepared from the cells, run on a formaldehydeagarose gel, transferred to a Nylon membrane, and hybridized with probes of various cytokines as described in Materials and Methods...