Escherichia coli BL21(DE3)pLysS competent cells (Promega) were transformed with the appropriate pGEX vectors, and protein expression was induced by culturing the cells with 1 mM IPTG for 6 h at 30 °C. Bacterial cells were collected and lysed in the B-PER reagent (Thermo Fischer Scientific)....
even simple linear Gaussian models can have estimation problems Marie Auger-Méthé1, Chris Field1, Christoffer M. Albertsen2, Andrew E. Derocher3, Mark A. Lewis3,4, Ian D. Jonsen5 & Joanna Mills Flemming1 State-space models (SSMs) are increasingly used in ecology...
DC-SIGN has shown to mediate binding of SARS-CoV pseudo-typed vectors to human DC with uptake into endosomes. The virus is then delivered via an infection synapse, which is also observed be- tween DC and T cells in studies investigating DC-SIGN involvement in the pathogenesis of HIV [237]...
Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 1992, 116, 43–49. [CrossRef] 80. Horna, D.H.; Gómez, C.; Olano, C.; Palomino-Schätzlein, M.; Pineda-Lucena, A.; Carbajo, R.J.; Braña, A.F.; Méndez, C.; Salas,...
27. Vectors New Zealand. Available online: https://vectors.co.nz/wpcontent/uploads/Blank_Map_Of_New_ Zealand.png (accessed on 4 May 2017). 28. Land Information New Zealand (LINZ). Available online: https://data.linz.govt.nz/layer/88131-northland- 04m-rural-aerial-photos-2014-16/ (...