If Biden can shave off enough Republican voters in the suburbs and broader southeastern Wisconsin area, together with the heavily Democratic urban centers of Milwaukee and Madison, there aren’t enough Trump voters in the rest of the state to get him reelected. It’s a formula that other...
The majority of the songs are dance songs or songs that are very uptempo. Very little in the way of singer/songwriter types. I like things that kept the mood up and had some spirit to them. The lyrical content could be kinda down, but the overall package had to be up. So you’ll ...
PCR was performed on DNA extracted by Wizard-purification (Promega, Madison, USA) from HeLa cell cultures infected with either C. trachomatis A, B, C, D or L2 and treated with Proteinase K for one hour at 55 °C. PCR reactions were performed with the ExpandTM High Fidelity PCR System ...
Additionally, yeast viability was tested using a CaspACE™ FITC-VAD-FMK In Situ Marker (Promega, Madison, WI, USA) according to the manufacturer’s protocol. Briefly, a dye at the final concentration of 10 µM in PBS was added to the cells and incubated for 30 min. Subsequently, the...
Methods of Soil Analysis: Part 2-Chemical and Microbiological Properties; ASA SSSA: Madison, WI, USA, 1965. [Google Scholar] Walkley, A.; Black, I.A. An examination of the Degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration ...
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The Textile Trade of Seventeenth-Century Northern Coromandel: A Study of a Pre-Modern Asian Export Industry; The University of Wisconsin-Madison: Madison, WI, USA, 1975. [Google Scholar] Fleming, L.E.; Maycock, B.; White, M.P.; Depledge, M.H. Fostering human health through ocean ...
Plasmid transfection was performed using FuGene® HD Transfection Reagent (Promega, Madison, WI, USA; HeLa cells) or Lipofectamine® 2000 (Invitrogen, Waltham, MA, USA; IPO13+/+ and IPO13−/− mESCs) according to manufacturer's instructions. The Dharmacon ON- TARGETplus siRNA system (...
Control Renilla luciferase construct pRL-SV40 (0.1 µg; Promega, Madi- son, WI, USA) and Firefly luciferase reporter construct (1 µg) were co-transfected into cells with GeneJuice (Novagen, Madison, WI, USA). Luciferase activity was measured using a Dual-Glo luciferase assay system (...