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MCF-7 cells were resuspended into a complete growth medium, plated into a 96-well microplate (~10,000 cells per well), and maintained in a serum-containing medium for >12 h until the cells adhered to the wall of the microplate wells. After the MCF-7 cells were treated with liposomal CA...
rings (compounds #1, #2 and #3) were generally more effective in downregulating HSPA1 than those with 2-carbon oxyethylene linker (compounds #4 and #5). Its presence was crucial for gene downregulation in Caco-2 cells. Of the evaluated analogues, compounds #2 and #3 seem to be the ...