For enzymes that have multiple substrates, such as proteases, the catalytic efficiency is also referred to as the specificity constant for a substrate. Explain how this number can reflect the substrate preference of the enzyme. The ...
The method comprises the steps of introducing the set of genes into a heterologous host cell, allowing growth of the cells in a suitable medium such that the expression of the genes results in production of enzymes. When specific substrate(s) is/are provided to the transformed cell, the ...
Enzymatic mechanisms are typically inferred from structural data. However, understanding enzymes require unravelling the intricate dynamic interplay between dynamics, conformational substates, and multiple protein structures. Here, we use single-molecule nanopore analysis to investigate the catalytic conformational...
Harnessing Conformational Plasticity to Generate Designer Enzymes 2020, Journal of the American Chemical Society Single-mutation fitness landscapes for an enzyme on multiple substrates reveal specificity is globally encoded 2017, Nature Communications In vitro-selected drug-resistant varicella-zoster virus mutan...
Plots show abundances of genes coding for carbohydrate-active enzymes (CAZymes) with relevance for degradation of two selected glycan substrates in the genomes of marine bacteria (at the top of each panel) and their expression during the bloom (at the bottom of each panel). Selected substrates ...
The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on...
In mammals, these reactions are catalyzed on multiple substrates by a family of nine protein arginine methyltransferases (PRMTs). However, conditions that may regulate the activity of each enzyme and that may help us understand the physiological role of PRMTs have not been fully established. ...
In all domains of life, DNA ligases are essential enzymes, catalyzing the joining of breaks in nucleic acid backbones for genome replication and repair. These enzymes are essential components in in vitro DNA manipulation procedures, playing a critical role in applications like cloning, sequencing, ...
In addition to the functions of TAMs, the contents of cytokines, enzymes, and growth factors and the composition of the ECM in the TME can be defined. The scavenging function of TAMs can control the concentration of growth factors and ECM regulatory components via active scavenger receptor-mediat...
It has been reported that enzymes matrix metalloproteinases 9 (MMP9) and matrix metalloproteinases 3 (MMP3) are involved in the cleavage of pro-HBEGF to form s-HBEGF.37,38 Interestingly, we also observed that sorafenib could increase both MMP9 and MMP3 protein levels in a transcription-depen...