为了进一步研究MPOA CaMKIIα+神经元和LHCaMKIIα+神经元的功能联系,采用了纤维光度法,将AAV-hSyn-DIO-ChrimsonR-mCherry注射到MPOA中,激活MPOA CaMKIIα+神经元(图4 e1);同时,用AAV-EF1α-DIO-GCaMP6s感染LH CaMKIIα+神经元(图4...
如图10所示，是发表在《Nature Neuroscience》杂志上题为“Circuit specificity in the inhibitory architecture of the VTA regulates cocaine-induced behavior”的文章，通过将AAV-DIO-ChR2-YFP病毒注射VGAT-IRES-Cre小鼠，实现了VGAT启动子驱动的Cre依赖的ChR2的特异性表达。图10. ChR2在小鼠多巴胺神经元中特异性...
Using a DIO system in combination with a low expression of Cre, Clover was sparsely expressed in neurons and was excited by two-photon excitation at 1000 nm. b, c Two-photon images of dendritic spines of layer 2/3 pyramidal neurons of the sensory cortex. The paCaMKII was activated by ...