Calu-3 cells are a human airway epithelial cell line that exhibits secretory characteristics similar to airway gland epithelial cells when grown on Transwell membrane filters. QRT-PCR analysis revealed that Calu-3 cells express mRNA for multiple Toll receptor (TLR) subtypes including TLR1, TLR2, ...
ObjectiveTo establish an HPLC assay to determine the uptake characteristics of bencycloquidium bromide (BCQB) in Calu-3 cells. Methods The samples were analyzed with a Discovery Ccolumn (4.6 mm×250 mm, 5μm) at 25 ℃. The mobile phase was acetonitrile-water containing 0.27% KHPO and 0.3...
Calu-3细胞高效液相色谱法摄取ObjectiveTo establish an HPLC assay to determine the uptake characteristics of bencycloquidium bromide (BCQB) in Calu-3 cells. Methods The samples were analyzed with a Discovery Ccolumn (4.6 mm×250 mm, 5μm) at 25 ℃. The mobile phase was acetonitrile-water ...
Presents evidence that Calu-3 human lung epithelial cells in culture, display vectorial dimeric immunoglobulin A (dIgA) transport modulated by the inflammatory mediator interferon-gamma (IFN-gamma). Characteristics of Calu-3 monolayers; Expression of secretory component messenger RNA; Preferential apical ...
As the usually used filter supports hindered particle transport due to their small pore size, supports with a pore size of 3 渭m had to be used. On those filters, barrier and transport characteristics of the cells were tested and culture conditions changed to obtain optimal conditions. ...