Plug in and turn on the spectrophotometer. Allow it to warm up for 15 minutes. Press the Percent T/A selector to select Percent Transmittance or Percent Absorbance mode. Locate the wavelength dial beside the sample chamber and set it to the desired wavelength. Don gloves and wipe a cuvette ...
from Chapter 4 / Lesson 5 45K Beer's law expresses the relationship between light intensity and the concentration of a substance through a formula. Learn the background of light transmittance and absorbance, and how the equation of Beer's law is used in science. Related...
What is the transmittance if you measure the potassium chromate on the preset conditions at 411 nm and 100 muM? A. 10.94% B. 63.14% C. 33.34% D. 29.44% How does the following error in technique lead to to incorrect absorbance readings and there...
What is the wavelength of light emitted when an electron falls from the n=6 to n=1 level in a H-atom? What is the transmittance at a given wavelength if 90% of the light at that wavelength is absorbed? A laser emits a l...